|
KMID : 0829320090120010048
|
|
Korean Journal of Clinical Microbiology
2009 Volume.12 No. 1 p.48 ~ p.52
|
|
|
A Case of Emetic Toxin Producing Bacillus cereus Strains Isolated from Outbreak
|
|
Kim Jong-Hyun
Park Mi-Sun
Lee Bok-Kwon
Lim Eun-Gyoung
Jang Hyun-Chul
Park Ju-Young
Lee Sun-Jin
Choi Gil-Bae
|
|
|
Abstract
|
|
|
|
Bacillus cereus causes two types of gastrointestinal diseases: emesis and diarrhea. It produces one emetic toxin and nine different enterotoxins. In March 2008, eight of a family became sick after eating slices of raw fish. We isolated emetic toxin producing B. cereus from the stools of 6 patients and 2 subclincal humans. In this study, the presence of enterotoxin genes, such as those of haemolysin BL (Hbl), nonhemolytic enterotoxin (Nhe), B. cereus enterotoxin T (BceT), enterotoxin FM (EntFM), cytotoxin K (cytK) and cereulide were assayed by polymerase chain reaction(PCR) methods. Their enterotoxin activities were assayed using the BCET- RPLA, Tecra ELISA kit and Hep-2 vacuole activity. Bacterial isolates were subtyped by pulsed-field gel electrophoresis (PFGE). This study demonstrates the emetic toxin-producing stains of B. cereus in clinical specimens, for the first time in the Republic of Korea.
|
|
|
KEYWORD
|
|
|
Emetic toxin, Cereulide, B. cereus
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|